Administration of substances and blood sampling

 Recommended needle size 

       sc - subcutaneously 
       im - intra muscle 
       iv - intra vein 
       ip - intra peritoneally

Mouse
   sc: 25 G     im: 27 G       iv: 26-28 G   ip: 25-27 G   

Rat
   sc: 25 G      im: 25 G       iv: 25-27 G    ip: 23-25 G   

Rabbit
   sc: 21-25 G     im: 25 G    iv: 23-25 G    ip: 21-23 G  

 

Diehl et al: A GOOD PRACTICE GUIDE TO THE ADMINISTRATION OF SUBSTANCES AND REMOVAL OF BLOOD, INCLUDING ROUTES AND VOLUMES – Journal of Applied Toxicology 21, 15 - 23, 2001

For blood sampling instructions please visit the National Centre for the 3Rs of Animal Research.

https://www.nc3rs.org.uk/our-resources/blood-sampling