Cell and Tissue Imaging Unit

 

Our imaging core facility provides the instruments to image biological processes from tissues to single cells and subcellular molecular kinetics with both conventional and high-throughput methods. We offer personal user training and support for imaging of fixed or live tissues or cells, including consultation on experimental set up, sample preparation and image processing and analysis. The services include a modern histology laboratory equipped with modern devices and services.

Institute of Biomedicine

Confocal microscopes are located in Snellmania Building, 3rd floor, room Sn 3151

IncuCyte S3 imaging platform is located in Snellmania Building, 3rd floor, room Sn 3182/2

Contact

Janne Capra (Core Manager), janne.capra(at)uef.fi, p 050-5165268, room Sn 3209

Kirsi Ketola (IncuCyte Expert), kirsi.ketola (at) uef.fi, p 050-3299984, room Sn 3210

Kirsi Rilla (Director of the Unit), kirsi.rilla (at) uef.fi, p 040-3553218, room Sn 3214/2

Equipment

ZEISS LSM700 (quick guide)

Basic confocal microscope for both live and fixed cell and tissue samples.
Inverted microscope with 10 x, 20 x, 25 x water/oil, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm

ZEISS LSM800 Airyscan (quick guide)

Superresolution microscope for imaging of live and fixed samples with high resolution and sensitivity.
Inverted microscope with 10 x, 20 x, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm.
Airyscan imaging mode for increased resolution and signal-to-noise ratio

INCUCYTE S3 (reservation instructions)

High-throughput imaging station for live-cell and time-lapse experiments.
Six plates for simultaneous automated acquisition and analysis of living cells
Incubator with temperature and CO2 control
CMOS camera with 4 x, 10 x and 20 x objectives
Image acquisition using visible light, green and red fluorescence
Modules for Standard imaging, Image Lock, Scratch Wound, Whole Cell, Dilution Cloning, Spheroid, Chemotaxis, Angiogenesis and Neurotracker.

Workstation
With ZEN software for image analysis.

Olympus IX71

Widefield fluorescence microscope equipped with a microinjection device and relief contrast technology.

ZEN SOFTWARE
To view and process your images ZEN freeware is available in https://www.zeiss.com/microscopy/int/downloads.html.
Fill the form and you will get the link for installation via email.

Reservations

University Outlook web access calendar:

ZEISS LSM700:

e-kuo-bio-zeiss_lsm700 (at)uef.fi

ZEISS LSM800 Airyscan:

e-kuo-bio-airyscan (at)uef.fi

INCUCYTE S3:

e-kuo-bio-incucyteplate

Each plate (1-6) has a separate calendar.
When booking a plate, check other plates as well to make sure your experiment fits!

Prices

  Mon-Fri 8-16 Mon-Fri 16-8, weekends Non-university users
LSM 700 12 €/h 6 €/h 20 €/h
LSM 800 Airyscan 20 €/h 12 €/h 30 €/h
IncuCyte S3 2 €/Gb of data 2 €/Gb of data 2 €/Gb of data

Links

Cell and Tissue Imaging Unit's Yammer page

SIB Labs

Biocenter Finland Biological imaging platform

Zeiss LSM

How to properly acknowledge the UEF Cell and Tissue Imaging Unit

All work performed at the UEF Cell and Tissue Imaging Unit should be properly acknowledged. This includes all microscopy, sample preparation, data processing, expert help, etc. performed here.

Why to acknowledge: A proper acknowledgment provides a visible measure of the impact of the UEF Cell and Tissue Imaging Unit on research, and secures development of the infrastructure and helps to keep the user fees at a reasonable level.. Also, -core facility personnel are scientists. When they make a substantial intellectual or experimental contribution to a publication they deserve to be acknowledged just as any other co-author. It also helps the personnel to advance in their careers.

When to acknowledge: Any time the UEF Cell and Tissue Imaging Unit provides services that support your research (including your usage of any equipment). When a staff member of the UEF Cell and Tissue Imaging Unit has made a significant intellectual contribution beyond a routine analysis, please consider co-authorship as well. 

Where to acknowledge: Papers, posters, presentations, all scholarly publications.

How to acknowledge: If the participation of the core personnel is not significant enough to warrant a co-authorship, a mention in the Acknowledgements section of the manuscript is sufficient. Please make sure that the "UEF Cell and Tissue Imaging Unit" is always mentioned correctly. For example: This work/Part of the work was carried out with the support of UEF Cell and Tissue Imaging Unit, University of Eastern Finland, Finland.