Cell and Tissue Imaging Unit

 

Our imaging core facility provides the instruments to image biological processes from tissues to single cells and subcellular molecular kinetics with both conventional and high-throughput methods. We offer personal user training and support for imaging of fixed or live tissues or cells, including consultation on experimental set up, sample preparation and image processing and analysis. The services include a modern histology laboratory equipped with modern devices and services.

Institute of Biomedicine

Confocal microscopes are located in Snellmania Building, 3rd floor, room Sn 3151

IncuCyte S3 imaging platform is located in Snellmania Building, 3rd floor, room Sn 3182/2

Contact

Kirsi Rilla (director of the unit), kirsi.rilla (at) uef.fi, p 040-3553218, room Sn 3214/2
Kirsi Ketola (IncuCyte manager), kirsi.ketola (at) uef.fi, p 050-3299984, room Sn 3210

Janne Capra (confocal microscope manager), janne.capra(at)uef.fi, p 050-5165268, room Sn 3209

Equipment

ZEISS LSM700 (quick guide)

Basic confocal microscope for both live and fixed cell and tissue samples.
Inverted microscope with 10 x, 20 x, 25 x water/oil, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm

ZEISS LSM800 Airyscan (quick guide)

Superresolution microscope for imaging of live and fixed samples with high resolution and sensitivity.
Inverted microscope with 10 x, 20 x, 40 x oil and 63 x oil –objectives
Incubator with temperature and CO2 control
Four lasers available: 405, 488, 555 and 640 nm.
Airyscan imaging mode for increased resolution and signal-to-noise ratio

INCUCYTE S3 (reservation instructions)

High-throughput imaging station for live-cell and time-lapse experiments.
Six plates for simultaneous automated acquisition and analysis of living cells
Incubator with temperature and CO2 control
CMOS camera with 4 x, 10 x and 20 x objectives
Image acquisition using visible light, green and red fluorescence
Modules for Standard imaging, Image Lock, Scratch Wound, Whole Cell, Dilution Cloning, Spheroid, Chemotaxis, Angiogenesis and Neurotracker.

Workstation
With ZEN software for image analysis.

Olympus IX71

Widefield fluorescence microscope equipped with a microinjection device and relief contrast technology.

ZEN SOFTWARE
To view and process your images ZEN freeware is available in https://www.zeiss.com/microscopy/int/downloads.html.
Fill the form and you will get the link for installation via email.

Reservations

University Outlook web access calendar:

ZEISS LSM700:

e-kuo-bio-zeiss_lsm700 (at)uef.fi

ZEISS LSM800 Airyscan:

e-kuo-bio-airyscan (at)uef.fi

INCUCYTE S3:

e-kuo-bio-incucyteplate

Each plate (1-6) has a separate calendar.
When booking a plate, check other plates as well to make sure your experiment fits!

Prices

  Mon - Fri 8 - 16         Mon - Fri 16 -8,  weekends
LSM700 15 €/h 10 €/h
LSM800 Airyscan      20 €/h 15 €/h

 

Links

SIB Labs

Biocenter Finland Biological imaging platform

Zeiss LSM